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anti cd43 magnetic beads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec anti cd43 magnetic beads
    Anti Cd43 Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd43+magnetic+beads/CD43+Glycoform+Antibody%2C+anti-mouse%2C+REAfinity/pm42013839-270-11-30
    Average 96 stars, based on 1 article reviews
    anti cd43 magnetic beads - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombination Assay:

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion
    Article Snippet: .. For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 10 5 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml −1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.
    Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 105 cells per ml in RPMImedium supplemented with 15% FBS and 25 ngml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Single Cell:

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion
    Article Snippet: .. For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 10 5 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml −1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.
    Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 105 cells per ml in RPMImedium supplemented with 15% FBS and 25 ngml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Magnetic Beads:

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion
    Article Snippet: .. For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 10 5 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml −1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Article Title: The KU70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of KU along DNA
    Article Snippet: .. The splenic CD43 − cells, isolated from 8–12 weeks old mice with CD43 magnetic beads (MACS, Miltenyi Biotec), were cultured in RPMI medium (Gibco) supplemented with 15% FBS, 1 μg/mL anti-CD40 (BD Pharmingen, 553721) and 20 ng/mL IL-4 (R&D, 404-ML-050) at a density of 1 × 10 6 cells/mL. .. Cells were analyzed by flow cytometry after staining for IgG1 (FITC anti-IgG1, BD Pharmingen 553443) and B220 (PE/Cy7 anti-B220, BioLegend 103222) on days 3 and 4.

    Article Title: Lactate Blocks Tertiary Lymphoid Structure Formation by Inhibiting B Cell Chemotaxis
    Article Snippet: For transwell migration assays, lymph node cells were cultured for 24 hours in migration medium (RPMI (Gibco) supplemented with 1% BSA (Sigma) and 10 mM HEPES (Gibco)) containing 30 ng/mL BAFF (Bio-techne). .. B cells were isolated by negative selection using CD43 magnetic beads (Miltenyi) and LD columns following the manufacturer’s protocol. .. Transwell migration assays were performed using 96-well plates with 5 μm pore inserts (Corning).

    Article Title: DIS3 licenses B cells for plasma cell differentiation in humans
    Article Snippet: .. Resting B cells were isolated using CD43 magnetic beads and LS columns on a QuadroMACSTM separator (Miltenyi Biotec). .. Cells were cultured at 1 × 10 6 cells/mL in RPMI supplemented with 10% fetal bovine serum, sodium pyruvate, non-essential amino acids, and penicillin/streptomycin (10 4 U/mL), stimulated with 1 ng/μL of LPS (Invivogen), and treated with 4 μM ASOs for 72 h. Cells were analyzed by flow cytometry based on CD138 (BD Biosciences) and B220 (BD Biosciences) expression (Table ).

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.
    Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 105 cells per ml in RPMImedium supplemented with 15% FBS and 25 ngml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Article Title: DIS3 licenses B cells for plasma cell differentiation in humans.
    Article Snippet: .. Resting B cells were isolated using CD43 magnetic beads and LS columns on a QuadroMACSTM separator (Miltenyi Biotec). .. Cells were cultured at 1 × 106 cells/mL in RPMI supplemented with 10% fetal bovine serum, sodium pyruvate, nonessential amino acids, and penicillin/streptomycin (104 U/mL), stimulated with 1 ng/μL of LPS (Invivogen), and treated with 4 μM ASOs for 72 h. Cells were analyzed by flow cytometry based on CD138 (BD Biosciences) and B220 (BD Biosciences) expression (Table 3).

    Article Title: TLR7/8 stress response drives histiocytosis in SLC29A3 disorders.
    Article Snippet: Splenic B cells were purified by negative selection using CD43 MicroBeads (Miltenyi Biotec). .. Splenocytes fromWT and Slc29A3−/− mice were labeled with CD43 magnetic beads and CD43-negative splenic B cells were enriched using autoMACS (Miltenyi Biotec) and subjected to experiments. .. Preparation of BM-derived macrophages and pDCs BM cells were collected from the tibiae, femora, and pelvises of WT, Slc29a3−/−, and Slc29a3−/−Tlr7−/− mice, and red blood cells were removed using BD Pharm Lyse lysing buffer.

    Article Title: TLR7/8 stress response drives histiocytosis in SLC29A3 disorders
    Article Snippet: Splenic B cells were purified by negative selection using CD43 MicroBeads (Miltenyi Biotec). .. Splenocytes from WT and Slc29A3 −/− mice were labeled with CD43 magnetic beads and CD43-negative splenic B cells were enriched using autoMACS (Miltenyi Biotec) and subjected to experiments. .. BM cells were collected from the tibiae, femora, and pelvises of WT, Slc29a3 −/− , and Slc29a3 −/− Tlr7 −/− mice, and red blood cells were removed using BD Pharm Lyse lysing buffer.

    Magnetic Cell Separation:

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion
    Article Snippet: .. For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 10 5 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml −1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Article Title: The KU70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of KU along DNA
    Article Snippet: .. The splenic CD43 − cells, isolated from 8–12 weeks old mice with CD43 magnetic beads (MACS, Miltenyi Biotec), were cultured in RPMI medium (Gibco) supplemented with 15% FBS, 1 μg/mL anti-CD40 (BD Pharmingen, 553721) and 20 ng/mL IL-4 (R&D, 404-ML-050) at a density of 1 × 10 6 cells/mL. .. Cells were analyzed by flow cytometry after staining for IgG1 (FITC anti-IgG1, BD Pharmingen 553443) and B220 (PE/Cy7 anti-B220, BioLegend 103222) on days 3 and 4.

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.
    Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 105 cells per ml in RPMImedium supplemented with 15% FBS and 25 ngml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Cell Culture:

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion
    Article Snippet: .. For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 10 5 cells per ml in RPMI medium supplemented with 15% FBS and 25 ng ml −1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Article Title: The KU70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of KU along DNA
    Article Snippet: .. The splenic CD43 − cells, isolated from 8–12 weeks old mice with CD43 magnetic beads (MACS, Miltenyi Biotec), were cultured in RPMI medium (Gibco) supplemented with 15% FBS, 1 μg/mL anti-CD40 (BD Pharmingen, 553721) and 20 ng/mL IL-4 (R&D, 404-ML-050) at a density of 1 × 10 6 cells/mL. .. Cells were analyzed by flow cytometry after staining for IgG1 (FITC anti-IgG1, BD Pharmingen 553443) and B220 (PE/Cy7 anti-B220, BioLegend 103222) on days 3 and 4.

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.
    Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 105 cells per ml in RPMImedium supplemented with 15% FBS and 25 ngml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Isolation:

    Article Title: The KU70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of KU along DNA
    Article Snippet: .. The splenic CD43 − cells, isolated from 8–12 weeks old mice with CD43 magnetic beads (MACS, Miltenyi Biotec), were cultured in RPMI medium (Gibco) supplemented with 15% FBS, 1 μg/mL anti-CD40 (BD Pharmingen, 553721) and 20 ng/mL IL-4 (R&D, 404-ML-050) at a density of 1 × 10 6 cells/mL. .. Cells were analyzed by flow cytometry after staining for IgG1 (FITC anti-IgG1, BD Pharmingen 553443) and B220 (PE/Cy7 anti-B220, BioLegend 103222) on days 3 and 4.

    Article Title: Lactate Blocks Tertiary Lymphoid Structure Formation by Inhibiting B Cell Chemotaxis
    Article Snippet: For transwell migration assays, lymph node cells were cultured for 24 hours in migration medium (RPMI (Gibco) supplemented with 1% BSA (Sigma) and 10 mM HEPES (Gibco)) containing 30 ng/mL BAFF (Bio-techne). .. B cells were isolated by negative selection using CD43 magnetic beads (Miltenyi) and LD columns following the manufacturer’s protocol. .. Transwell migration assays were performed using 96-well plates with 5 μm pore inserts (Corning).

    Article Title: DIS3 licenses B cells for plasma cell differentiation in humans
    Article Snippet: .. Resting B cells were isolated using CD43 magnetic beads and LS columns on a QuadroMACSTM separator (Miltenyi Biotec). .. Cells were cultured at 1 × 10 6 cells/mL in RPMI supplemented with 10% fetal bovine serum, sodium pyruvate, non-essential amino acids, and penicillin/streptomycin (10 4 U/mL), stimulated with 1 ng/μL of LPS (Invivogen), and treated with 4 μM ASOs for 72 h. Cells were analyzed by flow cytometry based on CD138 (BD Biosciences) and B220 (BD Biosciences) expression (Table ).

    Article Title: DIS3 licenses B cells for plasma cell differentiation in humans.
    Article Snippet: .. Resting B cells were isolated using CD43 magnetic beads and LS columns on a QuadroMACSTM separator (Miltenyi Biotec). .. Cells were cultured at 1 × 106 cells/mL in RPMI supplemented with 10% fetal bovine serum, sodium pyruvate, nonessential amino acids, and penicillin/streptomycin (104 U/mL), stimulated with 1 ng/μL of LPS (Invivogen), and treated with 4 μM ASOs for 72 h. Cells were analyzed by flow cytometry based on CD138 (BD Biosciences) and B220 (BD Biosciences) expression (Table 3).

    Selection:

    Article Title: Lactate Blocks Tertiary Lymphoid Structure Formation by Inhibiting B Cell Chemotaxis
    Article Snippet: For transwell migration assays, lymph node cells were cultured for 24 hours in migration medium (RPMI (Gibco) supplemented with 1% BSA (Sigma) and 10 mM HEPES (Gibco)) containing 30 ng/mL BAFF (Bio-techne). .. B cells were isolated by negative selection using CD43 magnetic beads (Miltenyi) and LD columns following the manufacturer’s protocol. .. Transwell migration assays were performed using 96-well plates with 5 μm pore inserts (Corning).

    Staining:

    Article Title: ATR kinase supports normal proliferation in the early S phase by preventing replication resource exhaustion.
    Article Snippet: .. Class switch recombination and cell trace violet staining For the Class Switch Recombination assay, single-cell suspensions of spleen cells were sorted with CD43 magnetic beads (MACS, Miltenyi), and CD43- B cells were cultured at a density of 5 × 105 cells per ml in RPMImedium supplemented with 15% FBS and 25 ngml−1 of IL-4 (R&D) and anti-CD40 (BD Biosciences). ..

    Labeling:

    Article Title: TLR7/8 stress response drives histiocytosis in SLC29A3 disorders.
    Article Snippet: Splenic B cells were purified by negative selection using CD43 MicroBeads (Miltenyi Biotec). .. Splenocytes fromWT and Slc29A3−/− mice were labeled with CD43 magnetic beads and CD43-negative splenic B cells were enriched using autoMACS (Miltenyi Biotec) and subjected to experiments. .. Preparation of BM-derived macrophages and pDCs BM cells were collected from the tibiae, femora, and pelvises of WT, Slc29a3−/−, and Slc29a3−/−Tlr7−/− mice, and red blood cells were removed using BD Pharm Lyse lysing buffer.

    Article Title: TLR7/8 stress response drives histiocytosis in SLC29A3 disorders
    Article Snippet: Splenic B cells were purified by negative selection using CD43 MicroBeads (Miltenyi Biotec). .. Splenocytes from WT and Slc29A3 −/− mice were labeled with CD43 magnetic beads and CD43-negative splenic B cells were enriched using autoMACS (Miltenyi Biotec) and subjected to experiments. .. BM cells were collected from the tibiae, femora, and pelvises of WT, Slc29a3 −/− , and Slc29a3 −/− Tlr7 −/− mice, and red blood cells were removed using BD Pharm Lyse lysing buffer.



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